129.
Alicyclobacillus acidocaldarius
MEDIUM
Solution A:
Yeast extract 1.00 g
(NH
4
)
2
SO
4
0.20 g
MgSO
4
.7H
2
O 0.50 g
CaCl
2
.2H
2
O 0.25 g
KH
2
PO
4
0.60 g
Glucose 1.00 g
Distilled water 500 ml
Adjust pH to 3.0-4.0
Solution B:
Agar powder 20.00 g
Distilled water 500 ml
Sterlize solution A and B separately at 121°C for 15 min. Cool to 50°C and then combine.
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