• Casamino acids 5.00 g
• Glucose 2.00 g
• KH₂PO₄ 0.86 g
• K₂HPO₄ 0.55 g
• Sodium citrate 0.60 g
• CaCl₂ 0.10 g
• MnCl₂.4H₂O 0.016 g
• MgCl₂.6H₂O 0.43 g
• ZnCl₂ 7.00 mg
• FeCl₃ 3.00 mg
• Distilled water 1 L

Adjust pH to 6.5.

Commercially available Casamino acids may be used (Difco 0230, Oxoid L41, Pronadisa 1604).