• NaNO₃ 0.50 g
• K₂HPO₄ 1.00 g
• MgSO₄.7H₂O 0.50 g
• KCl 0.50 g
• FeSO₄.7H₂O 0.01 g
• Agar powder (only for solid medium) 15.00 g
  
• Distilled water 1 L
• Filter paper strips, sterile
  

Dissolve salts in distilled water and adjust pH to 7.2. Distribute the medium in tubes and add a strip of Whatman n°1 filter paper to each. The strip must not be completely submerged in the medium.

When used as solid agar (15 g/L) medium, sterilize the filter paper strips separately and place them aseptically on the surface of the solidified medium.

For Cellulomonas and Cellvibrio the addition of 0.5 g/L yeast extract is recommended.