• Yeast extract 0.5 g
• Proteose peptone nº3 0.5 g
• Casamino Acids 0.5 g
• Glucose 0.5 g
• Soluble starch 0.5 g
• Sodium pyruvate 0.3 g
• K₂HPO₄ 0.3 g
• Magnesium sulfate 0.05 g
• Agar powder 12.0 g
• Double distilled water 1 L

pH 7.2

Alternatively, commercially available medium (Difco 218263, EMD 1.00416) can be used.

If a broth medium is desired, it can be prepared component by component without adding agar. A commercial medium such as HiMedia M1687 can also be used.