150.
Thiobacillus
sp. MEDIUM
Solution A:
(NH
4
)
2
SO
4
0.40 g
KH
2
PO
4
0.20 g
MgSO
4
.7H
2
O 0.08 g
Distilled water 400 ml
Solution B:
FeSO
4
.7H
2
O 10 g
H
2
SO
4
(1N) 1 ml
Distilled water 99 ml
Dissolve all the ingredients of solution A and sterilize at 115°C for 30 min. Sterilize the solution B by filtration. Mix them when cool and dispense aseptically in tubes. Final pH to 2.8 with H
2
SO
4
(1N).
Imprimir