• NaCl 200.00 g
• KCl 1.00 g
• Na₂CO₃ 5.00 g
• NH₄Cl 1.00 g
• MgSO₄.7H₂O 0.24 g
• CaSO₄.2H₂O 0.17 g
• KH₂PO₄ 1.00 g
• Disodium glutamate 1.00 g
• Trace elements SL-10 1 ml
• Yeast extract 5.00 g
• Casamino acids 5.00 g
• Agar powder (only for solid medium) 20 g
• Distilled water up to 1 L

Adjust pH to 6.5 before autoclaving. Sterilize Na₂CO₃ separately and mix after cooling. Check final pH to 9.0-9.5.

Trace elements solution SL-10

• HCl (25%) 10 ml
• FeCl₂.4H₂O 1.5 g
• ZnCl₂ 70 mg
• MnCl₂.4H₂O 100 mg
• H₃BO₃ 6 mg
• CoCl₂.6H₂O 190 mg
• CuCl₂.2H₂O 2 mg
• NiCl₂.6H₂O 24 mg
• Na₂MoO₄.2H₂O 36 mg
• Distilled water 990 ml