161. GLYCEROL-SOIL MEDIUM

Peptone 5 g Beef extract 3 g Glycerol 20 g Soil extract 150 ml Tap water 850 ml Agar powder 15 g Adjust pH to 7.0. Soil extract is prepared by sieving air dried garden soil through a coarse sieve and autoclaving 400 g with 960 ml of tape water at 121°C for one hour. After the mixture is cool and settled, the supernatant is carefully decanted, filtered through paper, autoclaved in 200 ml quantities, and stored at room temperature until cleared by sedimentation. Imprimir