• CaCl₂ 0.20 g
• FeCl₂ 3.58 mg
• MnCl₂ 218.00 mg
• Standard medium 1 L

• NaCl 250 g
• MgSO₄.7H₂O 20 g
• Sodium citrate 3 g
• Peptone 10 g
• Distilled water 1 L

Adjust pH to 7.2.

For solid medium add 15 g of agar powder.