• Special peptone 23.0 g
• Starch 1.0 g
• NaCl 5.0 g
• Agar powder 10.0 g
• Distilled water 1 L

This recipe corresponds to dehydrated culture medium Oxoid CCM 0331. Alternativelly, other commercially available media are Difco 211124, Pronadisa 1104, ...

Medium 5 is recommended for reactivation of freeze-dried cells from the ampoule.