• KH₂PO₄ 1.36 g
• Na₂HPO₄ 2.13 g
• (NH₄)₂SO₄ 0.50 g
• MgSO₄.7H₂O 0.20 g
• CaCl₂.2H₂O 6.0 mg
• FeSO₄.7H₂O 3.0 mg
• MnSO₄.H₂O 1.0 g
• Na₂MoO₄.2H₂O 1.5 mg
• Methylamine hydrochloride 3.38 g
• Yeast extract 0.10 g
• Distilled water 1 L

Dissolve methylamine hydrochloride in 100 ml water. Dissolve all other ingredients in 900 ml water. Adjust pH to 7.4 with NaOH. Sterilize separately at 120ºC for 20 minutes. After autoclaving mix both solutions and dispense aseptically. Final pH 7.2.