• Trypticase soy broth 30.00 g
• Yeast extract 3.00 g
• NaCl 13.00 g
• KCl 0.34 g
• MgCl₂ x 6H₂O 4.00 g
• MgSO₄ x 7H₂O 3.45 g
• NH₄Cl 0.25 g
• CaCl₂ x 2H₂O 0.14 g
• Distilled water 1L

Adjust pH to 7.2.

Commercially available Trypticase soy broth (Difco 211768, Oxoid CM 129, Pronadisa 1224) can be used.