• Glucose 1.00 g
• Peptone 5.00 g
• Yeast extract 5.00 g
• Agar powder (only for solid media) 20 g
• Subov's salts solution 833 ml
• Distilled water up to 1 L

Adjust pH to 7.2.

Subov's salts solution (30%):

• NaCl 234.0 g
• MgCl₂ x 6H₂O 42.0 g
• MgSO₄ x 7H₂O 60.0 g
• CaCl₂ x 2H₂O* 1.1 g
• KCl 6.0 g
• NaHCO₃* 0.2 g
• NaBr 0.7 g
• FeCl₃ x 6H₂O (5% watery solution) 9 drops
• Distilled water up to 1 L