• Casein peptone 10.0 g
• Beef extract 3.0 g
• Yeast extract 3.0 g
• Glucose 2.0 g
• Tween 80 1 ml
• Cysteine hydrochloride 0.5 g*
• Salt solution 40 ml
• Distilled water 960 ml

Adjust pH to 7.2. For solid media add 15 g/L of agar powder.

Salt solution:

• CaCl₂ 0.2 g
• MgSO₄.7H₂O 0.2 g
• K₂HPO₄ 1.0 g
• KH₂PO₄ 1.0 g
• NaHCO₃ 10.0 g
• NaCl 2.0 g
• Distilled water 1 L

* After autoclaving add asseptically 10 ml of a 5 % (w/v) solution of cysteine hydrochloride sterilized by filtration.