199. Iron-Manganese-Thiosulphate-Citrate MEDIUM

NaCl 19.60 g Na2SO4 3.30 g KCl 0.50 g KBr 0.05 g H3BO3 0.02 g MgCl2·6H2O 8.80 g NaNO3 1.00 g Na2S2O3·5H20 5.00 g Yeast extract 0.02 g PIPES buffer 1.00 g Sodium citrate 1.00 g Agar powder (only for solid media) 13.00 g Distilled water up to 1L Autoclave, then aseptically add: FeSO4·7H2O (0.1% solution) 2.00 ml MnSO4·H2O 0.15 g CaCl2·2H2O 10.00 ml Trace Elements F solution 10.00 ml N/P solution 10.00 ml Adjust to pH 7.0. The ingredients to add after autoclaving can be combined together and added via filter sterilization. The iron sulphate solution must be made immediatly before use. Trace Elements F Al2(SO4)3 0.05 g H3BO3 0.10 g LiCl 0.05 g MnCl2·4H2O 0.20 g Na2MoO4·2H2O 0.10 g KBr 0.05 g KI 0.05 g NaF 0.05 g VOSO4·2H2O 0.0005 g ZnSO4·7H2O 0.10 g BaCl2 0.005 g CoCl2·6H2O 0.005 g CuSO4·5H2O 0.01 g NiCl2·6H2O 0.01 g Na2SeO4 0.005 g SrCl2·6H2O 0.005 g H2WO4 0.005g Distilled water up to 1L N/P Solution (NH4)2SO4 43.00 g KH2PO4 3.60 g NaNO3 60.3 g Distilled water up to 1L Imprimir