• NH₄Cl 0.30 g
• K₂HPO₄ 0.30 g
• MgSO₄.7H₂O 0.50 g
• CaCl₂.2H₂O 0.05 g
• KCl 0.30 g
• Na-acetate 1.00 g
• Yeast extract 1.00 g
• Bacto peptone 1.00 g
• Trace elements solution SL-6 (see medium 196) 1.00 ml
• Vitamin B₁₂ 20.00 μg
• Distilled water 1 L

Adjust pH to 7.5-7.8. For solid media add 15 g/L of agar powder. After autoclaving, add vitamin B₁₂ from a filter sterilized stock solution.