To prepare 1 L of medium and prevent precipitation, prepare solutions A, B, C and D, separately.

Solution A

• Na₂HPO₄.2H₂O 0.89 g
• NaNO₃ 5.00 g
• Agar powder (only for solid media) 15.00 g

• NaCl 23.00 g
• KCl 0.75 g
• CaCl₂.2H₂O 1.47 g

• MgSO₄.7H₂O 6.16 g
• MgCl₂.6H₂O 5.08 g

• FeSO₄.7H₂O 0.03 g
• Sodium pyruvate 10.00 g

Adjust each solution to pH 7.5 with NaOH 10%. Autoclave, cool at room temperature and mix.