• Glucose 10.0 g
• Peptone 5.0 g
• Yeast extract 5.0 g
• KH₂PO₄ 1.0 g
• MgSO₄.7H₂O 0.2 g
• NaCl 80.0 g
• Na₂CO₃ 20.0 g
  

For solid media add 20 g/L of agar powder.

Important: NaCl and Na₂CO₃ are autoclaved separately and added to the medium at  60ºC.