• Sucrose 103.00 g
• K₂SO₄ 0.25 g
• MgCl₂.6H₂O 10.12 g
• Glucose 10.00 g
• Casaminoacids 0.10 g
• Trace element solution 2 ml
• Yeast extract 5.00 g
• TES buffer 5.73 g
• Distilled water up to 1 L

Pour 100 ml of the solution into 250 ml Erlenmeyer flasks each containing 2.2 g agar. Close the flasks and autoclave. At time of use, re-melt the medium and supplement with the following ingredients in the order listed:

• KH₂PO₄ (0.5%) 1 ml
• CaCl₂.2H₂O (5 M) 0.4 ml
• L-proline (20%) 1.5 ml
• NaOH (1 N) 0.7 ml