31.
Rhizobium
sp. MEDIUM I
Yeast extract 1 g
Mannitol 10 g
Agar powder 15 g
Distilled water 800 ml
Soil extract 200 ml
Adjust pH to 7.0.
Soil extract:
Garden soil 77 g
Na
2
CO
3
0.2 g
Distilled water 200 ml
Autoclave soil suspension for 1 hour at 121 ºC. Filter through filter paper before add to the medium.
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