• Calf brain infusion solids 12.5 g
• Beef heart infusion solids 5.0 g
• Proteose peptone 10.0 g
• Glucose 2.0 g
• NaCl 5.0 g
• HNa₂PO₄ 2.5 g
• Distilled water 1 L

For solid media (BHA-Brain Heart Agar) add 15 g/L of agar powder.