51. Lactobacillus homohiochii MEDIUM

Tryptone 10 g Yeast extract 5 g Beef extract 2 g Glucose 20 g KH2PO4 0.5 g K2HPO4 0.5 g MgSO4.7H2O 0.2 g FeSO4.7H2O 10 mg MnSO4.H2O 7.5 mg Tween 80 1 ml Ethanol 40 ml Sodium acetate 20 g Distilled water 1 L Dissolve all ingredients in 950 ml of distilled water. Adjust pH to 5.2. Distribute in 9.5 ml amounts in tubes. Sterilize and add aseptically 0,4 ml of ethanol and 0.1 ml of a 3 mg/ml solution of DL-mevalonic acid. Imprimir