55. LOWENSTEIN-JENSEN MEDIUM

Several commercial brands are available (Difco 220908 and others; Scharlau 064-TA0165).

To the best of our knowledge there are none equivalent liquid media. For rehydration of freeze-dried cells, sterile saline solution is recommended.

Formula of Löwenstein-Jensen medium (per 600 mL):

  • KH2PO4 2.50 g
  • MgSO4 0.24 g
  • Mg-citrate 0.60 g
  • Asparagine 3.60 g
  • Potato flour 30.00 g
  • Malachite green 0.40 g
  • Distilled water 600 mL

Add:

  • Glycerol 12 mL

Mix thoroughly. Heat with agitation just until the medium boils. Autoclave at 121°C for 15 minutes. Cool to approximately 50°C, and add egg mixture gently.

Egg mixture:

Prepare 1000 mL of whole eggs. Clean eggs with ethanol, collect yolk and white aseptically, and mix thoroughly.

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