• Yeast extract 5.00 g
• Mycopeptone 10.00 g
• Adenine 0.01 g
• Uracil 0.01 g
• Succinic acid 10.00 g
• NaOH 6.00 g
• Sodium lactate 30.00 g
• Yeast Nitrogen Base 6.70 g
• Agar powder (only for solid medium) 15.00 g
• Distilled water 1 L

Important: Sterilize the Yeast Nitrogen Base by filtration. 

Vegetable peptone No. 1 can be used in place of mycopeptone.