85. Leuconostoc oenos MEDIUM (MLO)
  • Tryptone 10.00 g
  • Yeast extract 5.00 g
  • Glucose 10.00 g
  • Fructose 5.00 g
  • MgSO4.7H2O 0.20 g
  • MnSO4.H2O 0.05 g
  • Diammonium citrate 3.50 g
  • L-Cysteine hydrochloride 0.50 g*
  • Filtered tomato juice 100 ml
  • Tween 80 1 ml
  • Distilled water 900 ml
Adjust pH to 4.8.
For solid medium dissolve the ingredients in half of the volume required and adjust pH to 4.8. In the remaining water dissolve 20 g/L of Agar powder. Sterilize separately at 115°C for 25 min. Mix and dispense aseptically when still hot.

 

* After autoclaving add asseptically 10 ml of a 5 % (w/v) solution of cysteine hydrochloride sterilized by filtration.  



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