99. Rhodospirillaceae MEDIUM

KH2PO4 0.50 g MgSO4.7H2O 0.20 g NaCl 0.40 g NH4Cl 0.40 g CaCl2.2H2O 0.05 g Succinic acid 1.00 g Yeast extract 0.20 g Fe-citrate solution (0.10 g/100 ml) 5 ml Trace element solution SL-7 1.00 ml Cyanocobalamin (Vitamin B12) (1 mg/100 ml) 1.00 ml Distilled water 1 L Trace elements solution SL-7: HCl (25%) 1 ml ZnCl 70 mg MnCl2.4H2O 100 mg H3BO3 60 mg CoCl2.6H2O 200 mg CuCl2.2H2O 20 mg NiCl2.6H2O 20 mg NaMoO4.2H2O 40 mg Distilled water 1 L Adjust pH to 6.8. Dispense in screw cap tubes and sterilize at 120°C for 30 min with loose caps. The tubes are tightly closed after cooling at room temperature and can be stored for several months. Imprimir